PNC-27 5MG

Description

Champion Peptides PNC-27 5MG: Premium Research-Grade Cellular Targeting Peptide

Access new pathways in cellular research with Champion Peptides PNC-27 5MG. We design this compound for solid stability, precise target binding, and high purity. Get clear laboratory data. Use a peptide made for serious scientific study.

Premium PNC-27 5MG by Champion Peptides: High-Grade Cellular Ligands

Cellular biology moves fast. Oncology and therapeutic studies demand stable, clean reagents. There is no room for errors. Champion Peptides offers our lyophilized PNC-27 5MG to meet these high standards. It fits the needs of universities, private labs, and independent teams. If you want to buy PNC-27 online, our product provides great stability and clean, reliable data.

This synthetic chimeric peptide draws real interest. It targets abnormal cells in a specific way. The design pairs a p53 tumor suppressor protein binding domain with a membrane-penetrating vector. It is a useful tool. Researchers use it to study cell-cycle control, membrane transport, and cell lysis.

Ordering PNC-27 online from us gives you more than a simple chemical. You get a verified, stable research tool. Every single batch goes through clear testing—including High-Performance Liquid Chromatography (HPLC) and Mass Spectrometry (MS). This means you get reproducible data.

Understanding the Molecular Mechanism: How PNC-27 Interacts with Cellular Pathways

To see how this compound works, look at its physical makeup. It is a chimeric molecule with two key amino acid parts:

• The Target-Binding Domain: This part uses residues 12 to 26 of the p53 tumor suppressor protein. It binds naturally to the HDM2 protein, which normally degrades p53.
• The Membrane-Penetrating Domain (Penetratin): To get the peptide through the cell wall, the p53 sequence links to a 16-amino-acid carrier peptide. This comes from the Antennapedia homeodomain. We call it penetratin.

Researchers focus heavily on how this peptide interacts with cell membranes. Healthy cells keep HDM2 inside the nucleus and cytoplasm. In abnormal or rapidly growing cells, things change. HDM2 often moves to the outer cell membrane.

When you introduce high-quality PNC-27 to these cell models, the penetratin part helps the peptide slide right into the lipid bilayer. Once inside, the active part binds directly to membrane-bound HDM2. This bond changes the structure. It tears open the cell membrane, forming physical pores.

Most cancer drugs trigger programmed cell death—apoptosis—through complex paths. Sick cells often bypass this through mutation. PNC-27 works differently. It causes fast, physical cell death. It literally pops the cell membrane—a process called membranolysis—leading to necrosis. This physical pathway makes our premium PNC-27 highly useful for studying drug-resistant cells.

Product Specifications and the 5MG Lyophilized Advantage

When comparing the PNC-27 price and choosing a source, look at how the peptide is stored. Champion Peptides ships PNC-27 as a stable, freeze-dried powder. It comes in a vacuum-sealed 5MG vial.

Freeze-drying is a smart preservation step. It pulls out moisture while keeping the molecular shape intact. This stops early breakdown. It prevents water damage during shipping and long-term storage.

Technical Data Sheet

• Product Name: PNC-27
• Active Substance: p53-derived peptide (residues 12-26) linked to penetratin
• Total Quantity: 5 Milligrams (5MG) per vial
• Physical Form: Freeze-dried white powder
• Purity Level: Over 99.0% verified by HPLC and Mass Spectrometry
• Molecular Formula: C188H293N53O44S
• Sequence: Pro-Pro-Leu-Ser-Gln-Glu-Thr-Phe-Ser-Asp-Leu-Trp-Lys-Leu-Leu-Arg-Gln-Ile-Lys-Ile-Trp-Phe-Gln-Asn-Arg-Arg-Met-Lys-Trp-Lys-Lys
• Storage Requirements: Keep dry at -20°C for long-term storage; keep cold at 2°C to 8°C after mixing.
• Application: For laboratory research and in vitro/in vivo studies only. Not for human or animal use.

The 5MG size works perfectly for standard lab tests. It gives you enough peptide for several cell culture runs or animal micro-dosing. You do not waste material as you might with larger bottles. If you want to buy PNC-27 with a good balance of cost and purity, this 5MG vial makes perfect sense.

Key Features and Scientific Benefits of Champion Peptides PNC-27

• Ultra-High Purity Profile: Our process yields a peptide that is over 99% pure. We remove side products and solvents. Your cells will not face unexpected variables.
• Verified Structural Stability: The link between the p53 binding domain and the penetratin sequence is chemically confirmed. The membrane-penetrating action stays fully active when mixed.
• Optimal Freeze-Drying: Our freeze-drying process keeps the peptide safe at room temperature during transport. It holds its shape until you mix it.
• Selective Target Binding: The peptide binds tightly to membrane-bound HDM2. This makes it an exceptional choice for studying membrane damage without affecting healthy pathways.
• Consistent Batches: We use automated systems alongside human checks. Every vial you order online performs just like the last one. Your published data stays reliable.

Laboratory Reconstitution and Handling Protocols

To protect your PNC-27 5MG peptide, you must follow strict mixing and handling rules. Peptides are fragile. Physical force, heat, and bacteria can easily ruin them.

Reconstitution Liquid Selection

For cell work, use sterile Bacteriostatic Water (0.9% benzyl alcohol) or Sterile Water for Injection (WFI). If your test is sensitive to benzyl alcohol, sterile phosphate-buffered saline (PBS) works. Keep an eye on the pH so the peptide does not clump.

Reconstitution Method

1. Temperature Balance: Let the vial reach room temperature (20°C to 25°C) before opening. This stops moisture from forming inside, which destroys the powder.
2. Clean the Vial: Wipe the rubber top with an isopropyl alcohol swab. Let it dry.
3. Add Liquid: Use a clean, small syringe. Draw the exact amount of liquid you need. For instance, adding 1mL of water to a 5MG vial gives a concentration of 5mg/mL.
4. Inject Slowly: Push the needle through the center of the rubber top. Aim the needle at the glass wall, not the powder. Slowly run the liquid down the side to protect the peptide.
5. Swirl Gently: Remove the needle. Gently swirl the vial in a circle. Do not shake the vial. Shaking breaks the fragile peptide bonds. It ruins the compound. Swirl until the liquid is clear and the powder is gone.

Storage Guidelines for Reconstituted PNC-27

Mixed peptide breaks down quickly. Store the liquid in a fridge at 2°C to 8°C. Use it within 14 to 21 days for best results. For longer storage, split the liquid into single-use tubes and freeze them at -20°C or -80°C. Avoid freezing and thawing them repeatedly—this breaks the molecular structure.

Why Champion Peptides is the Leading Source for Research Peptides

The research chemical market is full of suppliers. It is hard to know where to buy PNC-27 with real confidence. Champion Peptides stands out. We focus on transparency, accurate science, and strict quality checks.

There is no room for error in lab work. One bad vial can ruin months of effort, waste funding, and spoil your data. That is why we run multi-stage tests. Our biochemists watch every step—from basic synthesis to final packing.

We offer fair, clear PNC-27 pricing while keeping our standards high. When you buy PNC-27 online from us, you get purity that matches or beats major global brands. It fits your budget. Our support team can help you with bulk orders, shipping, and technical questions, making the process simple.

Reference Details and Frequently Asked Questions

What is the primary difference between PNC-27 and PNC-28? These two chimeric peptides come from different parts of the p53 tumor suppressor protein. PNC-27 uses residues 12-26 of p53, while PNC-28 uses residues 17-26. Both connect to the penetratin sequence and target HDM2. But they have slight differences in binding strength, weight, and how they insert into the membrane. Researchers study them together to compare how they break down cell walls.

Can PNC-27 be used for in vivo animal studies? Yes. Researchers use PNC-27 in animal models like mice or rats to study how it moves through the body, where it goes, and its local effects. When planning animal studies, calculate the dose based on the animal’s size and the way you deliver it. Because it is a peptide, normal blood enzymes can clear it quickly. Localized delivery is common in research.

How should the lyophilized powder be stored upon arrival? Store the dry vials in a freezer at -20°C or colder. This keeps the dry peptide stable for up to 24 months. Keep it away from light and dampness to avoid early breakdown.

Why is the peptide delivered as a lyophilized powder instead of a liquid? Liquid peptides degrade fast. Water causes a chemical reaction called hydrolysis, which snaps peptide bonds. Liquid solutions also attract bacteria and spoil in warm temperatures. Freeze-drying pulls out the water. This leaves the peptide in a stable, dry state that travels and stores safely.

Does Champion Peptides provide third-party testing results? Yes. We believe in open data. Every batch of PNC-27 we sell undergoes independent testing. We can share HPLC and Mass Spectrometry reports on request to prove the purity of your batch.

Is PNC-27 safe for human use or clinical applications? No. PNC-27 is strictly a research chemical. The FDA and other regulators have not approved it for human use, medical treatment, or diagnostic work. Only trained professionals should handle it in a controlled lab setting. We enforce this rule and will cancel orders if we suspect misuse.

How does the penetratin domain work to cross cell membranes? The penetratin sequence is a cell-penetrating peptide. It has a high charge from amino acids like arginine and lysine. These positive charges bind to the negative charges on the cell’s outer wall. This lets the peptide cross the membrane directly without using cellular energy. It gives the p53 part of PNC-27 easy access to its target.

Step Up Your Research with Champion Peptides

High-stakes research demands reliable tools. Choosing Champion Peptides means getting some of the cleanest PNC-27 available. Our focus on purity, structural checks, and safe storage means your lab gets exactly what it needs.

Study the potential of membrane-specific binding with a compound built for precision. Order PNC-27 online from Champion Peptides today. See the difference high purity makes in your lab work.